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PAP, Liquid Based, Reflex HPV

Patient Prep

Do not use vaginal lubricants, vaginal medications, vaginal contraceptives or douches within 48 hours prior to the exam. Avoid in sexual activity 24 hours prior to test.

Container Type

ThinPrep® or SurePath® vial

Specimen Type

Cervical, endocervical, vaginal

Collection Procedure

Print the first and last name of the patient on specimen container. Do not place or label the lid of the container. Obtain cervical/vaginal material. Run the collection device along the inside of the vial then, break off the tip of the collection device, into the vial. Place the container lid on tightly and shake vigorously.


Real-Time PCR - The Cobas HPV test is based on two major processes: (1) automated specimen preparation to simultaneously extract HPV and cellular DNA, (2) PCR amplification22 of target DNA sequences using both HPV and B-globin specific complementary primer pairs and real-time detection of cleaved fluorescent labeled HPV and B-globin specific oligonucleotide detection probes.

Reflex Testing

HPV performed if ASCUS 

Detection of 14 high-risk (HR) HPV types in a single analysis. The test identifies High Risk genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68 concurrently but does not specifically identify which genotype was detected.

Store, Transport and Stability

Room Temp

Room Temp SurePath®: 4 weeks; ThinPrep®: 18 weeks  

Day(s) Test Set Up

Mon - Sat

Turn Around Time

2-3 days



87624 as appropriate