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T-Cell Subset, Blood

Performing Laboratory

Providence Alaska Medical Center

Methodology

Flow Cytometry

Specimen Requirements

Specimen must arrive within 48 hours of draw.

 

Draw blood in a 3-mL, lavender-top (EDTA) tube; and send 3 mL (minimum volume:  1.5 mL) of EDTA whole blood at ambient temperature.

Reference Values

SURFACE MARKER

% T-cells (CD3)

0-2 months:  53-84%

3-5 months:  51-77%

6-11 months:  49-76%

12-23 months:  53-75%

2-5 years:  56-75%

6-11 years:  60-76%

12-17 years:  56-84%

≥18 years:  52-84%

% Helper cells (CD4)

0-2 months:  35-64%

3-5 months:  35-56%

6-11 months:  31-56%

12-23 months:  32-51%

2-5 years:  28-47%

6-11 years:  31-47%

12-17 years:  31-52%

≥18 years:  30-61%

% Suppressor cells (CD8)

0-2 months:  12-28%

3-5 months:  12-23%

6-11 months:  12-24%

12-23 months:  14-30%

2-5 years:  16-30%

6-17 years:  18-35%

≥18 years:  12-42%

ABSOLUTE COUNTS

T-cells (CD3)

0-2 months:  2,500-5,500 cells/µL

3-5 months:  2,500-5,600 cells/µL

6-11 months:  1,900-5,900 cells/µL

12-23 months:  2,100-6,200 cells/µL

2-5 years:  1,400-3,700 cells/µL

6-11 years:  1,200-2,600 cells/µL

12-17 years:  1,000-2,200 cells/µL

≥18 years:  582-1,992 cells/µL

Helper cells (CD4)

0-2 months:  1,600-4,000 cells/µL

3-5 months:  1,800-4,000 cells/µL

6-11 months:  1,400-4,300 cells/µL

12-23 months:  1,300-3,400 cells/µL

2-5 years:  700-2,200 cells/µL

6-11 years:  650-1,500 cells/µL

12-17 years:  530-1,300 cells/µL

≥18 years:  401-1,532 cells/µL

Suppressor cells (CD8)

0-2 months:  560-1,700 cells/µL

3-5 months:  590-1,600 cells/µL

6-11 months:  500-1,700 cells/µL

12-23 months:  620-2,000 cells/µL

2-5 years:  490-1,300 cells/µL

6-11 years:  370-1,100 cells/µL

12-17 years:  330-920 cells/µL

≥18 years:  152-838 cells/µL

LYMPHOCYTE RATIO

H/S ratio:  ≥1.0

Day(s) Test Set Up

Monday through Friday

Test Classification and CPT Coding

86360