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Lactate, Plasma

Performing Laboratory

Providence Alaska Medical Center

Methodology

Enzymatic End point

Specimen Requirements

Specimen must arrive in laboratory within 8 hours of draw.

 

Draw blood in a grey-top (potassium oxalate/sodium fluoride) tube(s). (Hemolyzed or serum specimen is not acceptable.) Spin down, separate plasma from cells within 15 minutes of draw, and send 2 mL (minimum volume - 1 mL) of potassium oxalate/sodium fluoride plasma.

Note:  Do not use stasis (tourniquet) or have patient pump their fist while drawing specimen.

 

Alternate sample:

Green top, lithium heprin, whole blood.  Put on ice immediately after blood draw and send 2 mL (minimum 1mL) to the lab.

Reference Values

Sample Type - Plasma, Potassium/Oxalate

      Venous: 0.5 - 2.2 mmol/L

      Arterial: 0.6 - 1.4 mmol/L

 

Sample Type - Whole blood, lithium heparin

      Venous: 0.90 - 1.70 mmol/L

      Arterial: 0.6 - 1.40 mmol/L

 

 

Note:  Values may be elevated 20% to 50% after meals.

Stability

Whole blood sample is stable for 1 hour on ice only.

 

If unable to deliver to the laboratory within 1 hour, centrifuge grey top tube (potasium oxalate sample) and remove plasma from red cells within 15 minutes of collection.  Plasma should be transferred to a clean, closed container and left anerobic. Stable at 15-25 deg C for 8 hours, or 14 days at 2-8 deg C.

Day(s) Test Set Up

Monday through Sunday

Available STAT

Test Classification and CPT Coding

83605

ETA

Estimated turnaround time 35 min

Critical Values

> 4.0 mmol/L

Critical values for this test will be called to the patient's provider.